Advances in synthetic biology have resulted in the development of genetic tools that support the design of complex biological systems encoding desired functions. The majority of efforts have focused on the development of regulatory tools in bacteria, whereas fewer tools exist for the tuning of expression levels in eukaryotic organisms. Here, we describe a novel class of RNA‐based control modules that provide predictable tuning of expression levels in the yeast Saccharomyces cerevisiae. A library of synthetic control modules that act through posttranscriptional RNase cleavage mechanisms was generated through an in vivo screen, in which structural engineering methods were applied to enhance the insulation and modularity of the resulting components. This new class of control elements can be combined with any promoter to support titration of regulatory strategies encoded in transcriptional regulators and thus more sophisticated control schemes. We applied these synthetic controllers to the systematic titration of flux through the ergosterol biosynthesis pathway, providing insight into endogenous control strategies and highlighting the utility of this control module library for manipulating and probing biological systems.
The design of complex biological systems encoding desired functions require the development of genetic tools for the precise control of protein levels in cells (Elowitz and Leibler, 2000; Gardner et al, 2000; Basu et al, 2004). For example, in the design of engineered metabolic networks, the tuning of enzyme levels is often critical for overcoming metabolic burden (Jones et al, 2000; Jin et al, 2003), the accumulation of toxic intermediates (Zhu et al, 2001; Pfleger et al, 2006) and detrimental consequences associated with the redirection of cellular resources from native pathways (Alper et al, 2005b; Paradise et al, 2008). Various examples of libraries of genetic control modules have been described that have been generated through the randomization of well‐characterized gene expression control elements (Basu et al, 2004; Pfleger et al, 2006; Anderson et al, 2007). However, most of these studies have been conducted in Escherichia coli such that there is a lack of similar tools for other cellular chassis.
The budding yeast, Saccharomyces cerevisiae, is a relevant organism in industrial processes, including biosynthesis and biomanufacturing strategies (Ostergaard et al, 2000; Szczebara et al, 2003; Nguyen et al, 2004; Veen and Lang, 2004; Ro et al, 2006; Hawkins and Smolke, 2008). The majority of existing methods for tuning gene expression in yeast are through transcriptional control mechanisms in the form of inducible and constitutive promoter systems (Hawkins and Smolke, 2006; Nevoigt et al, 2006; Nevoigt et al, 2007). RNA‐based control modules based on posttranscriptional mechanisms may offer an advantage in that they can be coupled to any promoter of choice, providing for enhanced control strategies and finer resolution tuning of protein expression levels. Although posttranscriptional control elements, such as internal ribosome entry sites and AU‐rich elements, have been applied to regulate heterologous gene expression in yeast (Vasudevan and Peltz, 2001; Zhou et al, 2001; Lautz et al, 2010), these control elements have exhibited substantial variability in activity and have not been engineered as synthetic libraries exhibiting a wide range of predictable gene regulatory activities.
RNase III enzymes are a class of enzymes that cleave double‐stranded RNA. The S. cerevisiae RNase III enzyme, Rnt1p, exhibits a number of unique features that allow it to recognize very specific RNA hairpin substrates that harbor a consensus AGNN tetraloop sequence. Despite extensive characterization of this enzyme and its demonstrated role in processing non‐coding RNA and mRNA, neither natural nor synthetic Rnt1p substrates have been used to control gene expression levels in yeast. Therefore, we developed a genetic control system based on directed Rnt1p processing of a target transcript. Specifically, Rnt1p hairpins were immediately flanked by a clamp sequence (that insulates the hairpin structure from surrounding sequences) and placed downstream of a gene of interest, where they direct cleavage and thus inactivate the transcript, resulting in rapid transcript degradation. We validated this Rnt1p‐based control system with two Rnt1p hairpins based on previous in vitro studies and demonstrated that Rnt1p hairpins can act as gene control modules in yeast.
Previous in vitro studies had identified three key regions in Rnt1p hairpins: the cleavage efficiency box (CEB), the binding stability box and the initial binding and positioning box (Lamontagne et al, 2003). The CEB region affects the processing of the hairpin stem by Rnt1p, such that nucleotide (nt) modifications in this region are expected to specifically modulate the cleavage rate. We created an Rnt1p hairpin library by randomizing the CEB region (12 nt). This library was placed downstream of a fluorescent reporter protein and a cell‐based screening assay was used to identify functional members of the library that resulted in lowered fluorescence levels. The functional Rnt1p hairpin library comprises 16 unique sequences that span a large gene regulatory range—from 8 to 85% (Figure 3A)—and are fairly evenly distributed across this range. The negative controls for each sequence (constructed by mutating the required consensus tetraloop sequence) demonstrated that the majority of gene knockdown observed from each hairpin is due to Rnt1p processing (Figure 3B). A correlation analysis on the transcript and protein levels for each library hairpin construct indicated a strong positive correlation and a strong preservation of rank order between the two in vivo regulatory measurements (Figure 3C). Characterization of the hairpin library in a different genetic context supported the broader utility of these control modules for providing predictable gene control.
We applied the Rnt1p control modules to titrating a key enzyme component of the endogenous ergosterol biosynthesis network—the ERG9 genetic target. Squalene synthase, encoded by the ERG9 gene, is responsible for catalyzing the conversion of two molecules of farnesyl pyrophosphate to squalene, the first precursor in the ergosterol biosynthetic pathway in S. cerevisiae (Poulter and Rilling, 1981; Figure 6A). We integrated several members of the Rnt1p hairpin library downstream of the native ERG9 gene to cover the regulatory range of the library (Figure 6B). A strong positive correlation and preservation of rank order was observed between the ERG9 transcript levels and their yEGFP3 counterparts (Figure 6C). However, ERG9 expression levels did not fall below ∼40%, regardless of the Rnt1p hairpin strength, indicating that a previously identified endogenous feedback mechanism associated with the native ERG9 promoter acts to maintain ERG9 expression levels at that threshold value. In addition, most strains exhibited high relative ergosterol levels and growth rates, except for two strains harboring synthetic Rnt1p hairpins that resulted in the lowest expression levels, which exhibited a significant reduction in the amount of ergosterol produced and growth rate (Figure 6D and E). Our studies indicate that the endogenous feedback mechanism can be acting to increase ERG9 expression levels to the desired set point in the slow‐growing strains, but the perturbations introduced in these strains may result in other impacts on the pathway that inhibit the endogenous control systems from restoring cellular growth to wild‐type rates. These studies support the unique ability of the synthetic Rnt1p hairpin library to systematically titrate pathway enzyme levels by introducing precise perturbations around major control points while maintaining native cellular control strategies acting through transcriptional mechanisms.
Rnt1p hairpins can act as effective posttranscriptional gene regulatory elements in the yeast Saccharomyces cerevisiae.
Modification of the cleavage efficiency box (CEB) region of an Rnt1p hairpin can modulate Rnt1p cleavage rates, and thus the resulting gene regulatory activities of the hairpin control elements.
A library of Rnt1p hairpins can act as a set of synthetic control modules that provide predictable tuning of gene expression over a wide range of expression levels.
The Rnt1p‐based control elements can be combined with any promoter to support titration of regulatory strategies encoded in transcriptional regulators, including feedback control around endogenous proteins.
Synthetic biology is advancing capabilities for engineering biological systems exhibiting desired functions. The proper functioning of synthetic genetic circuits often relies on precise control and tuning of the expression levels of key protein components. For example, the proper functioning of synthetic gene networks exhibiting complex dynamic behaviors has been shown to depend on the appropriate matching of levels of protein components in the engineered networks (Elowitz and Leibler, 2000; Gardner et al, 2000; Basu et al, 2004). The tuning of protein levels to obtain functioning circuits has been commonly achieved by screening randomized gene expression control elements for those sequences that provide the desired regulatory strength (Basu et al, 2004; Pfleger et al, 2006; Anderson et al, 2007). As another example, the optimization of engineered metabolic networks has been shown to depend on the precise control of enzyme levels and activities (Pfleger et al, 2006; Pitera et al, 2007; Peralta‐Yahya and Keasling, 2010). The tuning of enzyme levels is critical for reducing metabolic burden due to enzyme overexpression (Jones et al, 2000; Jin et al, 2003), decreasing accumulation of toxic intermediates by balancing pathway flux (Zhu et al, 2001; Pfleger et al, 2006), and redirecting cellular resources from native pathways without negatively affecting the health and viability of the engineered host by knocking out required enzymes (Alper et al, 2005b; Paradise et al, 2008). As such, the development of well‐characterized gene expression control modules that can be used to predictably tune the levels of proteins are key to the design of robust genetic systems.
Although many gene regulatory tools have been developed for use in Escherichia coli (Jensen and Hammer, 1998; Carrier and Keasling, 1999; Smolke et al, 2004; Alper et al, 2005a), fewer such tools exist for the precise tuning of expression levels in the budding yeast, Saccharomyces cerevisiae. However, S. cerevisiae is a relevant organism in industrial processes, including biosynthesis and biomanufacturing strategies (Ostergaard et al, 2000; Szczebara et al, 2003; Nguyen et al, 2004; Veen and Lang, 2004; Ro et al, 2006; Hawkins and Smolke, 2008), such that as more complex genetic networks are engineered into yeast, it becomes critical to have tools that allow for the facile programming of gene expression levels. The existing methods for tuning gene expression levels in S. cerevisiae rely on transcriptional control mechanisms in the form of inducible and constitutive promoter systems. Many inducible promoters do not provide tunable control systems because of their on/off switch‐like behavior, wherein the amount of inducer molecule controls the likelihood that a given cell is repressed or fully expressing the desired protein (Louis and Becskei, 2002). Although engineered variants have been constructed that offer more tunable responses (Hawkins and Smolke, 2006; Nevoigt et al, 2007), these systems can exhibit other undesirable properties because of nonspecific or pleotropic effects associated with the inducing molecule or limitations associated with costs in using the inducing molecule in large‐scale processes. As an alternative strategy, a promoter library was recently developed based on mutating the constitutive TEF1 promoter (Nevoigt et al, 2006). The resulting library of promoter parts comprised 11 promoter variants that spanned expression levels from 8 to 120%, providing a useful tool for controlling expression levels in yeast. However, control modules based on transcriptional mechanisms require the use of a particular promoter, which may be limiting to certain applications. For example, the use of a specific or native promoter may be desired to retain cellular control mechanisms associated with the given promoter. RNA‐based control modules based on posttranscriptional mechanisms may offer an advantage by allowing these control elements to be coupled to any promoter of choice, providing for enhanced control strategies and finer resolution tuning of expression levels.
Endoribonucleases have key roles in RNA processing across diverse cellular systems (Caponigro and Parker, 1996). In eukaryotic cells, endoribonuclease cleavage in the untranslated regions (UTRs) or in the coding regions of a transcript can result in rapid degradation of that transcript by exoribonucleases. The RNase III family is a class of enzymes that cleaves double‐stranded RNA (dsRNA; Filippov et al, 2000). The S. cerevisiae RNase III enzyme, Rnt1p, recognizes RNA hairpins that contain a consensus AGNN tetraloop and cleaves its substrates 14 nucleotides (nt) upstream and 16 nt downstream of the tetraloop (Lamontagne et al, 2003). Rnt1p harbors an RNase III domain and a dsRNA‐binding domain (dsRBD; Filippov et al, 2000), in which the AGNN tetraloop of an Rnt1p substrate forms a predetermined fold that is recognized by the dsRBD (Wu et al, 2004). Rnt1p is localized to the nucleus, where it has been shown to cleave cellular ribosomal RNA precursors, small nuclear RNAs, small nucleolar RNAs and messenger RNAs (Elela et al, 1996; Chanfreau et al, 1997, 1998; Catala et al, 2004). However, despite extensive characterization of this RNA processing enzyme, neither natural nor synthetic Rnt1p substrates have been used to control gene expression levels in yeast.
We have demonstrated that Rnt1p substrates can be used as effective posttranscriptional gene control modules when placed in the 3′ UTR of a target transcript. We used this Rnt1p regulatory construct with a cell‐based screening strategy to develop a library of synthetic Rnt1p substrates that exhibit a wide range of gene regulatory activities (spanning 8 and 85%) to tune Rnt1p processing efficiency. In vivo and in vitro assays demonstrate that the library of control elements modulate transcript and protein levels through variations of the Rnt1p processing efficiency. The library of Rnt1p elements was applied to predictably modulate flux through an endogenous ergosterol biosynthesis network through the direct integration of the synthetic components with an endogenous gene target, highlighting the broader utility of these synthetic control modules. The described Rnt1p substrate library provides a new set of control modules that can be used to predictably tune gene expression in yeast with any desired promoter.
Implementing Rnt1p hairpins as RNA‐based gene regulatory components
Rnt1p is an RNase III enzyme that cleaves consensus hairpin structures in S. cerevisiae. For a hairpin to be effectively recognized and cleaved by Rnt1p, it must have the following consensus elements: an AGNN tetraloop and four base pairs immediately below the tetraloop (Figure 1A). An Rnt1p substrate can be divided into three critical regions: the initial binding and positioning box (IBPB), comprising the tetraloop; the binding stability box (BSB), comprising the base‐paired region immediately adjacent to the tetraloop; and the cleavage efficiency box (CEB), comprising the region containing and surrounding the cleavage site (Lamontagne et al, 2003). The CEB has no reported sequence or structural requirements. Rnt1p will initially position itself, bind to the tetraloop and cleave the hairpin at two locations within the CEB, that is, between the fourteenth and fifteenth nts upstream of the tetraloop and the sixteenth and seventeenth nts downstream of the tetraloop. Most naturally occurring Rnt1p hairpins have been identified in non‐coding RNAs (ncRNAs), in which Rnt1p has a critical role in ncRNA processing (Elela et al, 1996; Chanfreau et al, 1997, 1998). Synthetic trans‐acting RNA guide strands were recently used to direct Rnt1p processing of a target ncRNA (Lamontagne and Abou Elela, 2007). Rnt1p hairpins have also been identified within the coding region of at least one endogenous yeast gene, MIG2, in which Rnt1p was shown to have a role in controlling expression levels of that gene (Ge et al, 2005). However, the ability of Rnt1p hairpins to function as genetic control modules in regulating the expression of heterologous genes has not been previously examined.
Source data for Figure 1D [msb20114-sup-0007-SourceData-S7.xls]
We designed a system that uses Rnt1p‐mediated hairpin cleavage to regulate gene expression in yeast through the modular insertion of Rnt1p hairpins in the UTRs of a gene (Figure 1B). Specifically, we inserted Rnt1p hairpins as gene control elements within the 3′ UTR of a transcript to direct cleavage to that region, thereby inactivating the transcript and resulting in rapid transcript degradation. Although directing cleavage to the 5′ UTR of a transcript would be expected to similarly inactivate the transcript, insertion of secondary structures in the 5′ UTR of eukaryotic transcripts has been shown to result in nonspecific translational inhibition due to affects of structural elements on ribosomal scanning (Pelletier and Sonenberg, 1985), such that resulting gene regulatory effects would likely not be specific to the desired cleavage mechanism. We designed and built a low‐copy Rnt1p characterization plasmid (pCS321) to quantify the gene regulatory properties of Rnt1p substrates in yeast. Unique restriction sites for inserting Rnt1p hairpins were located 2 nts downstream of the stop codon of a gene encoding a yeast‐enhanced green fluorescent protein (yEGFP3) (Mateus and Avery, 2000).
We first examined the ability of Rnt1p hairpins to function as gene control elements when placed downstream of a heterologous reporter gene in yeast. We adapted two Rnt1p hairpins with different CEBs, A01 and A02, that had been previously characterized through in vitro assays (R31‐27 and R31D, respectively, in Lamontagne and Elela, 2004; Figure 1C). The hairpins were modified by placing a G–C‐rich base‐paired region, or clamp, below the 18‐nt stem of the Rnt1p substrates to ensure structural stability of the hairpins when placed within the context of our Rnt1p characterization construct and to provide a proper stem length for effective cleavage in vivo. The hairpins were inserted into the characterization plasmid, and regulatory efficiencies were determined by monitoring cellular fluorescence by flow cytometry and transcript levels by quantitative real‐time PCR (qRT–PCR; Table I). Negative controls for Rnt1p hairpins were constructed by mutating the tetraloop sequence to CAUC to impede Rnt1p activity while maintaining the secondary structure of the hairpins. The fluorescence and transcript data for A01 and A02 show that nucleotide modifications in the CEB result in the attenuation of in vivo gene expression, and the mutated tetraloop controls support the fact that the observed regulatory effects are due to Rnt1p processing (Figure 1D). Flow cytometry histograms of the control hairpins demonstrate that regulatory activity causes a population shift with reduced median levels (Supplementary Figure S1A).
Design and selection of an Rnt1p cleavage library to achieve tunable gene regulatory control
Gene regulatory elements that allow the precise and predictable tuning of expression levels are important tools in synthetic biology for the control of gene circuits. The generation of well‐characterized libraries of gene control elements that exhibit varying regulatory properties has resulted in useful tools in bacteria (Jensen and Hammer, 1998; Carrier and Keasling, 1999; Alper et al, 2005a). Similar strategies have been applied to develop libraries of transcriptional control elements, specifically constitutive promoters, in yeast (Jeppsson et al, 2003; Nevoigt et al, 2006). However, certain applications will require circuit designs in which either native or inducible promoter systems will be desired, such that the ability to integrate posttranscriptional control elements that act downstream of desired promoter systems will be required. Libraries of tuned posttranscriptional stability control elements have not been developed to date in S. cerevisiae.
On the basis of the different gene regulatory activities observed in A01 and A02, we examined whether a larger library of synthetic Rnt1p hairpins could be engineered to develop a set of tuned posttranscriptional control elements. We developed an Rnt1p library based on randomizing the CEB (12 nt) to generate Rnt1p hairpins that exhibit different gene regulatory activities due to altered enzyme processing rates and identified synthetic Rnt1p substrates through a cell‐based fluorescence screen (Figure 2A). The designed library has a diversity of approximately 1.7 × 107 different hairpin sequences. Owing to the flexibility of the structural and sequence requirements for the CEB and the ability of each of the library members to bind Rnt1p through the maintained tetraloop structure, we anticipated that a large percentage of the library members would exhibit some cleavage activity. The goal of the functional screen of the library was to identify a set of Rnt1p hairpins that will provide a range of different regulatory activities and be useful as modular gene control elements.
The cleavage library was transformed into yeast through a gap‐repair strategy, and individual colonies were initially characterized for gene regulatory activity through assaying cellular fluorescence on a plate reader (Figure 2B). A total of 318 colonies were characterized, and from this initial screening, constructs from 41 low‐expressing colonies were sequenced (Supplementary Table SI). The sequences of the selected Rnt1p hairpins were analyzed by RNAstructure (http://rna.urmc.rochester.edu/RNAstructure.html) to determine the predicted secondary structure of the hairpins. No consensus secondary structure was identified from the 41 isolates because of the diversity of associated structures. The CEBs of the recovered hairpins are either completely base paired or contain one or two bulges of different size and location (Figure 2C). To ensure modularity of the synthetic Rnt1p hairpins to other genetic constructs, we removed library candidates that were ‘structurally weak’. Structurally weak hairpins were identified based on two properties: (1) predicted ability of the hairpin sequence to fold into multiple secondary structure conformations with similar free energies and (2) interactions with flanking sequences. In total, 16 Rnt1p cleavage library substrates were identified as synthetic control modules (Table I; Supplementary Figure S2).
A synthetic Rnt1p hairpin library exhibits a range of gene regulatory activities in vivo
The range of regulatory activities spanned by the cleavage library was measured at the protein expression and transcript levels. Flow cytometry analysis of the synthetic Rnt1p hairpins indicated that the selected set of hairpins spanned a large gene regulatory range—from 7.9% (C13) to 84.7% (C14) (Table I, Figure 3A). The regulatory activities of the selected hairpins are fairly evenly distributed across this range, allowing for precise tuning of expression levels based on insertion of different synthetic Rnt1p hairpins. Flow cytometry histograms of the library hairpins confirm that regulatory activity causes a population shift with reduced median GFP levels (Supplementary Figure S1B). The negative controls demonstrated that the majority of knockdown observed from each hairpin is due to Rnt1p processing (Figure 3B). The controls also indicate that the hairpin structures can have slight effects on gene expression (compared with the construct, with no hairpin insertion set at 100%), likely because of some effects of the inserted structures on normal translation or degradation processes.
Source data for Figure 3A [msb20114-sup-0001-SourceData-S1.xls]
Source data for Figure 3B [msb20114-sup-0002-SourceData-S2.xls]
Source data for Figure 3C [msb20114-sup-0003-SourceData-S3.xls]
The activity of the synthetic Rnt1p hairpins was further confirmed by monitoring steady‐state transcript levels in cells harboring the Rnt1p constructs (Table I, Figure 3B). Rnt1p hairpins generally resulted in reduced transcript levels compared with a construct harboring no Rnt1p hairpins and with a construct harboring a mutated tetraloop. In addition, a plot of normalized yEGFP3 expression levels versus normalized yEGFP3 transcript levels indicates that there is a strong positive correlation (r=0.817) between the two measures (Figure 3C). Specifically, with decreasing transcript levels, a similar decrease in protein levels was generally observed, as further supported from a Spearman's rank correlation coefficient (ρ) value of 0.818. Unintended effects of the hairpins on translation and transcript stability caused by interference of the structures on the machinery controlling those processes may contribute to deviations from linearity. However, observed deviations are not entirely due to structural variability between the library members, as hairpins with similar secondary structure (i.e., A01, A02, C13) do not demonstrate an exact linear relationship between transcript and protein levels.
Rnt1p library hairpins maintain regulatory activity in a different genetic context
The utility of any genetic control element requires that the control module retain its activity under different genetic contexts. The modular function of the synthetic Rnt1p hairpins, as measured by maintenance of gene regulatory activity, may be affected by differences in 3′ UTR, promoter and gene sequences. We cloned the synthetic Rnt1p hairpins into a second construct harboring a different promoter (TEF1), terminator (CYC1) and gene (ymCherry), and measured the regulatory activities of each hairpin through flow cytometry and qRT–PCR assays (Supplementary Table SII). The data indicate a strong positive correlation (r=0.897) and a strong preservation of rank order (ρ=0.882) between the ymCherry protein and transcript levels, confirming that gene regulatory activity by the Rnt1p modules is due to the reduction of steady‐state transcript levels (Figure 4A). Flow cytometry histograms confirm that regulatory activity associated with the Rnt1p hairpins in this second construct causes a population shift with reduced mean fluorescence levels (Supplementary Figure S3). The functional modularity of the hairpins was determined by performing a correlation analysis between expression data for the hairpins in the yEGFP3 and ymCherry constructs (Figure 4B), which demonstrated a strong positive correlation (r=0.856) between the two data sets. A lack of functional modularity was observed for one hairpin (C06), which did not maintain its gene regulatory activity in the second construct (33% yEGFP3 versus 89% ymCherry; Figure 4B; red point). The data suggests that the flanking sequences in the ymCherry construct may be disruptive to the structural integrity of C06, thereby affecting cleavage efficiency in the CEB, although RNA folding software does not predict alternative hairpin structures. The majority of data indicate that ymCherry expression tended to be slightly higher than that of yEGFP3, likely because of the differences in transcriptional strength between the TEF1 and GAL1 promoters, wherein TEF1 resulted in a greater absolute number of transcripts per cell (data not shown). The data indicate that there was a strong preservation of rank order (ρ=0.848) between the two different genetic contexts, supporting the functional modularity of the Rnt1p hairpin library.
In vitro characterization demonstrates that Rnt1p library members achieve differential activity through alterations in Rnt1p cleavage rates
We hypothesized that the variation in transcript processing and subsequent protein expression levels exhibited by the Rnt1p hairpin library is because of alterations of Rnt1p cleavage rates through alterations of the CEB sequence and/or structure. We analyzed the reaction through a Michaelis–Menten model, with the substrate (S) being the hairpin transcript, the enzyme (E) being Rnt1p and the product (P) being the cleaved pieces of the transcript. Under these conditions, the following reaction occurs:
The rate of product formation (V) is modeled as:
The maximum rate of product formation (Vmax) is the product of the total enzyme concentration ([E]0) and k2. Alterations in the cleavage efficiency will have an effect on the value of k2 and thus Vmax.
We performed in vitro RNA cleavage reactions with purified Rnt1p to determine relative values of k2 for each synthetic Rnt1p hairpin. Reactions were run with varying concentrations of in vitro synthesized radiolabeled RNA encoding an Rnt1p hairpin flanked by A‐rich sequences (see Materials and methods) and a constant concentration of purified Rnt1p. Reaction products were separated by denaturing polyacrylamide gel electrophoresis and quantified through phosphorimaging analysis (Figure 5A). The resulting data were fit to the Michaelis–Menten model to calculate a relative cleavage rate (RCR), which is directly proportional to Vmax. The RCR value for A01 is set to 1 and the rest of the reported values normalized to A01. The RCR values for each synthetic Rnt1p hairpin were determined through this analysis method (Table II). There is a direct relationship (r=−0.763) between the measured RCR and gene regulatory activity for the synthetic Rnt1p hairpins (Figure 5B). Specifically, increasing the ability of Rnt1p to cleave a substrate results in lowered transcript levels and thus lower protein expression levels. Notably, the transcript levels saturate at high RCRs, indicating that increasing the cleavage rates above a certain threshold results in limiting decreases in transcript levels. The trend saturates at ∼10% transcript levels, suggesting that increasing in vitro cleavage rates beyond an RCR value of ∼8 will not result in an increase in the amount of transcript being processed in vivo and that we have approached the maximum amount of knockdown that can be achieved with a single substrate in this system. In initial control tests, we found that the mutant tetraloop (CAUC) was cleaved in vitro under excessive protein concentrations (i.e., nine times greater than that used in the cleavage assay). As such, another mutant tetraloop (GAAA) that exhibited no cleavage under excessive protein concentrations in vitro was used as a control for the cleavage assays.
Source data for Figure 5B [msb20114-sup-0010-SourceData-S10.xls]
Source data for Figure 5D [msb20114-sup-0011-SourceData-S11.xls]
Although changes in the CEB are anticipated to result in changes to the Rnt1p processing efficiencies, it is also possible that the introduced sequence alterations may result in changes to the binding affinities between the hairpins and Rnt1p. To examine whether the synthetic Rnt1p hairpins exhibit any changes in binding affinity to Rnt1p, we performed in vitro binding assays with purified Rnt1p. Binding reactions were run with 20 nM of in vitro synthesized radiolabeled RNA encoding an Rnt1p hairpin and varying concentrations of purified Rnt1p in the absence of magnesium. As magnesium and other divalent metal ions are essential to Rnt1p function (Lamontagne et al, 2000), these reaction conditions allow for Rnt1p to bind to the substrates without subsequent cleavage. Bound products were separated by non‐denaturing polyacrylamide gel electrophoresis and quantified through phosphorimaging analysis (Figure 5C). We analyzed the reaction through a modified Scatchard equation in which the fraction of unbound RNA (R) to total RNA (R0) is plotted against the enzyme (E) concentration. The equation is as follows:
The dissociation constant, KD, for each synthetic Rnt1p hairpin was determined through this analysis method (Table II). The data indicate that there is no correlation between KD and in vivo gene regulatory activity (Figure 5D). The values of KD for the synthetic Rnt1p hairpins span a narrow range and are weakly correlated with transcript knockdown (r=0.351). A lack of correlation was anticipated because of expectations of mutations in the CEB primarily affecting cleavage. For a series of four hairpins with ∼70% transcript levels, the reported KD cover the range of the entire library, suggesting that nucleotide modifications in the CEB can have an effect on protein binding. The mutant tetraloop control binds with a similar KD as the library hairpins, although the binding is weaker than the library. While the mutant tetraloop does not affect binding greatly, it severely affects the ability of Rnt1p to bind in a conformation that allows cleavage, which has been previously reported (Lamontagne and Elela, 2004).
Control of endogenous ERG9 expression by 3′ UTR replacement with Rnt1p library members
In many metabolic engineering applications, there is a balance that must be maintained between diverting cellular metabolites to the production of desired compounds and the conversion of those metabolites to molecules required for cell growth and viability. In these cases, completely knocking out endogenous genes to remove the drain caused by native cellular pathways is not an option, and genetic tools that allow for precise titration of enzyme expression are desired such that flux through the endogenous pathway can be minimized to that required to maintain cell viability. Farnesyl pyrophosphate (FPP) is one such cellular metabolite that is an important precursor both to industrially relevant molecules and to molecules required for cell viability in yeast (Bach, 1995; Khosla and Keasling, 2003; Ro et al, 2006). Squalene synthase, encoded by the ERG9 gene, is responsible for catalyzing the conversion of two molecules of FPP to squalene, the first precursor in the ergosterol biosynthetic pathway in S. cerevisiae (Poulter and Rilling, 1981; Figure 6A). In a series of 14 catalytic steps, squalene is converted to ergosterol, the analog of cholesterol in mammalian cells. Ergosterol is an essential component of yeast cells because of its effect on structural stability of the cell membrane (Veen and Lang, 2004). Therefore, controlled reduction of ergosterol levels will allow metabolic flux to be diverted from sterol synthesis to value‐added products from FPP.
Source data for Figure 6C [msb20114-sup-0004-SourceData-S4.xls]
Source data for Figure 6D [msb20114-sup-0005-SourceData-S5.xls]
Source data for Figure 6E [msb20114-sup-0006-SourceData-S6.xls]
We examined the ability of our posttranscriptional genetic control modules to modulate flux through the ergosterol biosynthetic pathway in a predictable manner by incorporating several members of the Rnt1p library into the 3′ UTR of the ERG9 gene. ERG9 is in close proximity to CTF8 on the reverse strand of the chromosome (∼50 nts between stop codons), and there is a lack of information on the transcription terminators of both genes. Thus, we designed a construct to integrate the entire 3′ UTR and ADH1 terminator from the library plasmid (pCS321) immediately following the ERG9 stop codon and before the intervening nucleotides between ERG9 and CTF8 (Figure 6B). We built an Rnt1p control module integration plasmid based on the library plasmid, in which yEGFP3 was replaced by ERG9 and the marker loxP–KanMX–loxP (Guldener et al, 1996) was inserted downstream of the ADH1 terminator to provide resistance against G418. PCR amplification from the integration plasmid results in DNA cassettes that can be directly integrated into the desired location of the yeast genome. Six members of the synthetic Rnt1p library (A01, A02, C06, C07, C08 and C10) along with a mutant tetraloop control (C13, GAAA tetraloop) were integrated into the 3′ UTR of ERG9 to cover the regulatory range of the library.
The regulatory activity of the seven examined Rnt1p hairpins for ERG9 was initially assessed by measuring ERG9 transcript levels (Table III). The ERG9 transcript levels were directly compared with the transcript levels measured from the yEGFP3 construct. The Rnt1p hairpins resulted in reduced transcript levels compared with the integrant harboring no Rnt1p hairpins and with the construct harboring a mutated tetraloop. The transcript levels of the no hairpin integrant were higher than the transcript levels from the wild‐type yeast strain, indicating that the 3′ UTR replacement results in increased ERG9 transcript levels, likely due to altered transcript stability. In addition, a plot of the normalized yEGFP3 transcript levels versus normalized ERG9 transcript levels for each synthetic Rnt1p hairpin reveals a strong positive correlation (r=0.844) between the two measures when the hairpin C06 is excluded from the analysis, supporting the ability of the synthetic Rnt1p hairpins to act as predictable genetic control modules (Figure 6C). The modularity of the hairpin set (excluding C06) was further supported by a ρ‐value of 0.771, indicating a preservation of rank order. C06 was previously determined to not maintain function in the context of the ymCherry construct (Figure 4B; red point). The difference in regulatory activity observed from the C06 hairpin in the context of the endogenous ERG9 gene (Figure 6C; red point) further suggests that C06 may not be as well‐insulated from different genetic contexts as the other tested hairpins, although RNA folding software does not predict alternative structures. In addition, the ERG9 levels flatten and do not drop below ∼40%, indicating that the natural feedback regulation associated with the ERG9 promoter may act to maintain levels at this minimum value.
Although sterol synthesis is vital to cell growth, knockouts of enzymes in the downstream ergosterol biosynthetic pathway are viable because of the retained ability to incorporate intermediate sterols into the cellular membrane (Daum et al, 1998). However, knockouts of enzymes in the early part of the pathway, including ERG9 and enzymes leading up to the production of FPP, are lethal. As such, we examined the effect of decreased ERG9 expression on the cell growth rate. The OD600 of yeast strains harboring the different Rnt1p hairpins in the 3′ UTR of ERG9 was measured during the exponential growth phase. The growth rate, k, was calculated by fitting the OD600 data to an exponential growth curve (Table III). A plot of the growth rate versus ERG9 transcript levels for each strain reveals that above a certain threshold level of ERG9 production, differences in growth rates in this ‘fast‐growing’ regime are negligible (Figure 6D) This data suggest that decreasing the amount of ERG9 in this regime does not significantly affect the flux through the ergosterol biosynthetic pathway because of overproduction of ERG9 in the wild‐type strain or to feedback control of ERG9 (Kennedy et al, 1999). Below a certain threshold level, a ‘slow‐growing’ regime is observed, characterized by a substantial drop‐off in the cell growth rate. Interestingly, the two slow‐growing strains harbor the Rnt1p hairpins exhibiting the strongest gene silencing activities, but demonstrate similar ERG9 levels to two of the fast‐growing strains. It is possible that feedback regulation is acting to increase ERG9 expression to the desired set point in the slow‐growing strains, but the perturbations introduced in these strains result in other impacts on the pathway that inhibit the endogenous control systems from restoring cellular growth to wild‐type rates.
To better understand the cellular processes linking ERG9 production and cell growth rate, we measured the amount of ergosterol, the end product of the ERG9 biosynthetic pathway. Each culture was inoculated with the same amount of cells and allowed to grow for 8 h before the cultures were saponified. The UV spectrum of unsaponified sterols was determined and used to calculate a relative ergosterol value (REV), which is normalized against the control containing no Rnt1p substrate (set to 100%; Table III). A plot of REV versus ERG9 transcript levels reveals a similar relationship, as observed between growth rate and ERG9 levels, with the exception that the fast‐growing strains exhibit a slight positive correlation between REV and ERG9 transcript levels (Figure 6E). A plot of growth rate versus REV illustrates the two regimes (slow growing and fast growing) and highlights the strong positive correlation between these two phenotypic measures (r=0.953; Figure 6F). There is little difference in the amount of ergosterol per cell (determined as the ratio of REV and OD600), indicating that alteration of flux through the ERG9 pathway results in changes in the time required for a cell to produce sufficient ergosterol to duplicate and not decrease the levels of ergosterol molecules in the cell membrane.
We have developed a novel class of genetic control modules in S. cerevisiae based on Rnt1p cleavage. Although an Rnt1p substrate has been shown to have a role in regulating the expression of the endogenous MIG2 gene (Ge et al, 2005), our work describes the first synthetic gene regulatory system based on engineered Rnt1p hairpins. A library of synthetic Rnt1p hairpins that span a wide range of gene regulatory activities was generated to act as posttranscriptional control modules by placing these elements in the 3′ UTR of a target gene. To ensure the modularity of the synthetic Rnt1p substrates, two design strategies were implemented. First, a ‘clamp’ region was added to the base of each hairpin. Second, only sequences that formed single predicted hairpin structures at the lowest free energies were included within the Rnt1p control module set. These properties minimize any potential of the flanking sequences to disrupt the desired folding of the control modules, thereby reducing the likelihood of varying function within different genetic contexts. We observed a significant improvement in the correlation between transcript and protein levels for hairpins that exhibited both properties (Supplementary Figure S4), suggesting that undesired interactions between hairpin and flanking sequences can affect translation. The functional modularity of the resulting hairpin library, as measured by maintenance of regulatory activity and rank order, was demonstrated under three different genetic contexts (Figures 4B and 6C), wherein one hairpin (C06) did not retain its expected activity, likely because of improper folding. Our studies indicate that for any given genetic system, one of the library members (<10%) may not exhibit regulatory activity, in which case the coverage of the reported library will allow a researcher to select hairpins that span the desired regulatory range.
Previous in vitro studies identified three regions of the hairpin substrates to be critical to Rnt1p cleavage activity, namely, the CEB, BSB and IBPB (Lamontagne et al, 2003). The BSB and IBPB are regions of the hairpin that affect the overall binding of the protein such that modifications of the nucleotides in these regions can inhibit Rnt1p binding and subsequent cleavage. The CEB is a region of the hairpin that affects the processing of the stem by Rnt1p such that nucleotide modifications in this region are expected to specifically modulate the cleavage rate. We developed an Rnt1p library based on randomization of the CEB region and screened this library in vivo for substrates with altered processing efficiencies. Although earlier in vitro studies retained base pairing within the CEB of modified Rnt1p substrates (Lamontagne et al, 2003), our library screen demonstrated that the CEB has substantial structural flexibility in maintaining function in vivo, as the majority of library members retained processability. Cleavage assays with purified Rnt1p support that variations in the CEB within the set of synthetic Rnt1p substrates alter the processing efficiency in a manner that is directly correlated with the observed gene regulatory activity, whereas binding assays indicate no relationship between binding affinity and processing efficiency (or gene regulatory activity). The data support that modifications in the CEB directly influence the ability of Rnt1p to cleave the hairpin, whereas interactions between the nucleotides in the CEB and RBDs of Rnt1p likely lead to the small variation in observed KD values (Lamontagne et al, 2003).
The set of synthetic Rnt1p substrates developed in this work represents the first engineered library of transcript stability control modules in the yeast S. cerevisiae. Although other posttranscriptional regulatory elements, such as internal ribosome entry sites (IRESes) and AU‐rich elements, have been applied to regulate heterologous gene expression in yeast, such genetic elements have exhibited substantial variability in activity and have not been engineered as synthetic libraries of control modules exhibiting a wide range of activities (Vasudevan and Peltz, 2001; Zhou et al, 2001; Lautz et al, 2010). In addition, a library of short synthetic IRESes that act through translation initiation was previously developed for yeast (Zhou et al, 2003). However, these short IRESes result in substantially reduced expression levels compared with cap‐dependent translation mechanisms such that the resulting library spans a much narrower range of regulatory activities than exhibited by the synthetic Rnt1p library. The most similar control module library is one that was developed based on mutating a constitutive promoter (TEF1) in yeast, which spans a similar range of gene regulatory activities as the described Rnt1p hairpin library (TEF, 8–120%; Rnt1p, 2–100%; Nevoigt et al, 2006). However, the two libraries exhibit different coverage of these ranges, wherein the Rnt1p library provides greater coverage of expression levels between 2 and 60% and the TEF promoter library provides greater coverage between 60 and 100%.
A unique advantage of control modules based on posttranscriptional processes is that such elements can be readily used in combination with one another and with other genetic control modules, such as promoter elements and other transcriptional regulators, to achieve more finely tuned and expanded regulatory schemes. As one example, inducible promoters are commonly used to turn on and set the expression levels of genes by controlling the concentration of the inducing molecule exogenously added to the system. However, such transcriptional control modules on their own are limited to applying identical regulatory activities to multiple gene targets within a given system. The combination of an inducible promoter and our engineered Rnt1p substrates will allow for the relative gene regulatory activities at a given inducer concentration to be modulated (based on the Rnt1p substrate), thus enabling ligand‐mediated control over multiple genes with different expression levels.
In addition to expanding the utility of inducible promoters in the context of multi‐gene circuits, the ability to combine posttranscriptional control modules with any promoter element has key advantages in the context of endogenous networks. Endogenous networks often have critical control strategies in place, such as feedback regulation, that commonly operate at the level of transcriptional processes. The combination of the synthetic Rnt1p substrates with endogenous genetic targets allows specific engineered control strategies to be added to a system, while retaining native regulatory schemes that may have an important role in the overall system operation. Therefore, these posttranscriptional control modules provide a useful tool set for predictably modulating specific components in complex biological systems and can be further used to probe and study native regulatory networks. One consideration in the implementation of these genetic modules is that their gene regulatory activities may be affected by variation in the ratio of cellular levels of Rnt1p to transcript levels. Although absolute activities of the synthetic Rnt1p hairpins are expected to vary with substantial changes in Rnt1p levels, the rank order of the hairpin activities is expected to be maintained.
To demonstrate the utility of these posttranscriptional control modules, we implemented a synthetic control strategy directed to modulating a key enzyme component of the endogenous ergosterol synthesis network by combining the Rnt1p control modules with the ERG9 genetic target. Previous work had replaced the endogenous ERG9 promoter with a MET3‐repressible promoter, and demonstrated a sharp decrease in ergosterol levels with full transcriptional repression (Paradise et al, 2008). In contrast, our engineered control strategy was anticipated to allow the system to retain previously identified transcriptional feedback control around the ERG9 gene (Kennedy et al, 1999), while allowing for titration of ERG9 transcript levels. Generally, transcript levels between ERG9 and yEGFP3 with a given hairpin correlated strongly. However, relative ERG9 levels did not fall below ∼40%, regardless of the Rnt1p hairpin strength, indicating an endogenous feedback mechanism that maintains ERG9 expression levels at that threshold value. Interestingly, the data indicate a ‘buffer’ region in the endogenous control strategy around ERG9 levels, in which wild‐type levels are set substantially higher than that of threshold value. The synthetic Rnt1p hairpin set allowed for systematic titration of ERG9 expression levels and the identification of two regimes for the system. Strains expressing over ∼40% ERG9 transcript levels exhibited high ergosterol levels and growth rates. Strains harboring two synthetic Rnt1p hairpins resulting in the lowest expression levels exhibited a significant reduction in the amount of ergosterol produced and growth rate. Interestingly, these ‘slow‐growing’ strains have similar levels of ERG9 to two strains in the ‘fast‐growing’ regime. One possible explanation for these observations is that the diminished cellular sterol levels result in positive regulation of the ERG9 promoter below a certain threshold value, maintaining expression at a minimum level. However, dialing down ERG9 levels below a critical value can affect the cell through unknown mechanisms that do not permit restoration of ergosterol levels or cell growth rate through the endogenous control system (Kennedy et al, 1999). Therefore, this work supports the unique ability of the synthetic Rnt1p hairpin library to systematically titrate pathway enzyme levels while maintaining native cellular control strategies acting through transcriptional mechanisms.
In summary, we have developed a library of genetic control modules for yeast that can be implemented with different genetic targets and promoters to predictably tune gene expression levels. The Rnt1p library provides a key tool for synthetic biology applications in yeast, which can function to rationally dial in expression levels similar to well‐developed control modules in bacteria such as ribosome‐binding sites (RBS). However, unlike RBS elements, the structural and functional insulation of the synthetic Rnt1p controllers provide for more successful maintenance of regulatory activities across different genetic contexts (Salis et al, 2009). We show here that the synthetic controllers can be applied to predictably modulate flux through metabolic pathways and probe regulation schemes in endogenous networks by introducing precise perturbations around major control points. With growing interests in eukaryotic hosts and complex networks in synthetic biology, and more specifically in yeast in bioprocessing and biosynthesis applications, the synthetic controllers developed here will provide an important foundational tool set for the rapidly growing field.
Materials and methods
Standard molecular biology techniques were used to construct all plasmids (Sambrook and Russell, 2001). DNA synthesis was performed by Integrated DNA Technologies (Coralville, IA) or the Protein and Nucleic Acid Facility (Stanford, CA). All enzymes, including restriction enzymes and ligases, were obtained from New England Biolabs (Ipswich, MA) unless otherwise noted. Pfu polymerases were obtained from Stratagene. Ligation products were electroporated into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), and cells harboring cloned plasmids were maintained in Luria–Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). Clones were initially verified through colony PCR and restriction mapping. All cloned constructs and chromosomal integrations were sequence verified by Laragen (Los Angeles, CA) or the Protein and Nucleic Acid Facility (Stanford, CA). Plasmid maps are available in Supplementary Figure S5.
A yeast‐enhanced GFP gene, yEGFP3, was PCR amplified from pSVA13 (Mateus and Avery, 2000) using forward and reverse primers GFP.mono.di.fwd (5′‐GCAAGCTTGGAGATCTAAAAGAAATAATGTCT‐3′) and GFP.mono.rev (5′‐CGCTCGAGGCCTAGGCTTTATTTGTACAATT‐3′), respectively. The plasmid pCS182 was constructed by inserting the yEGFP3 PCR product into a modified version of pRS316 (Sikorski and Hieter, 1989) harboring the GAL1‐10 promoter via the unique restriction sites HindIII and XhoI located in the multiple cloning site (MCS) downstream of the GAL1‐10 promoter. The ADH1 terminator was PCR amplified from pSVA13 (Mateus and Avery, 2000) using the forward and reverse primers ADH1t_fwd (5′‐GCACCTCGAGAGGGCGCGCCACTTC‐3′) and ADH1t_rev (5′‐GCACGGTACCTATATTACCCTGTTATCCCTAGCGG‐3′), respectively. The base Rnt1p substrate characterization plasmid (pCS321) was constructed by inserting the ADH1 terminator PCR product into pCS182 via the unique restriction sites XhoI and KpnI located in the MCS. A ymCherry characterization plasmid, pCS1749, was constructed from pCS321 by replacing the GAL1‐10 promoter with the endogenous TEF1 promoter and by replacing the yEGFP3 open reading frame (ORF) and the ADH1 terminator with the ORF of ymCherry and the CYC1 terminator (J Liang et al, paper in preparation).
The endogenous S. cerevisiae gene RNT1 was PCR amplified directly from the yeast genome by colony PCR using forward and reverse primers Rnt1p_prmr_fwd (5′‐GACCATGGGGATGGGCTCAAAAGTAGCAGGTAAAAAGAAAACC‐3′) and Rnt1p_prmr_ rev (5′‐TGCTCGAGTCAGCTTGTATCTGAGAATTTTCTTTTCTTATTCTTTTGTGAG‐3′), respectively. The Rnt1p expression plasmid (pRNT1) was constructed by inserting the RNT1 PCR product into the pProEx HT plasmid (Stratagene) via the unique restriction sites NcoI and XhoI located in the MCS downstream of the 5′ (His)6 tag, the spacer region and rTEV protease cleavage site.
The endogenous S. cerevisiae gene ERG9 was PCR amplified directly from the yeast genome by colony PCR using forward and reverse primers ERG9_321_prmr_fwd (5′‐GCGAAGCTTGGAGATCTAAAAGAAATAATGGGAAAGCTATTACAATTGGCATTGCATCC‐3′) and ERG9_321_prmr_rev (5′‐GGCTCGAGGCCTAGGCTTCACGCTCTGTGTAAAGTGTATATATAATAAAACCCAAGAAGA‐3′), respectively. The plasmid pCS321‐ERG9 was constructed by replacing the yEGFP3 sequence in pCS321 with ERG9 by cloning the ERG9 PCR product into the unique restriction sites HindIII and XhoI. The plasmid pUG6 (Guldener et al, 1996) was modified by removing the unique XhoI restriction site by site‐directed mutagenesis via the oligonucleotides c1546g (5′‐GTGTCGAAAACGAGCTCTGGAGAACCCTTAATATAAC‐3′) and c1546g_antisense (5′‐GTTATATTAAGGGTTCTCCAGAGCTCGTTTTCGACAC‐3′) and the PfuUltra II polymerase (Stratagene). A PCR product harboring the full ERG9 coding sequence through the 3′ end of the ADH1 terminator was amplified from pCS321‐ERG9 using forward and reverse primers ERG9hpADH1t‐SalI_fwd_prmr (5′‐CAACGTCGACATGGGAAAGCTATTACAATTGGCA‐3′) and ERG9hpADH1t‐SalI_rev_prmr (5′‐AAGTGTCGACTATATTACCCTGTTATCCCTAGCGG‐3′), respectively. The ERG9‐RNT1 integration plasmid (pCS1813) was constructed by inserting this PCR product into the modified pUG6 plasmid via the unique restriction site SalI located directly upstream of the first loxP site.
Insertion of engineered Rnt1p substrates and appropriate controls into the 3′ UTR of pCS321, pCS1749 and pCS1813 was facilitated through either digestion with the appropriate restriction endonucleases and ligation‐mediated cloning or homologous recombination‐mediated gap‐repair during transformation into S. cerevisiae strain W303 (MATa, his3‐11,15 trp1‐1 leu2‐3 ura3‐1 ade2‐1) through standard lithium acetate procedures (Gietz and Woods, 2002). The Rnt1p substrates were amplified for insertion into pCS321 and pCS1813, with both techniques using the forward and reverse primers RntGap321_fwd (5′‐ACCCATGGTATGGATGAATTGTACAAATAAAGCCTAGGTCTAGAGGCG‐3′) and RntGap321_rev2 (5′ TAAGAAATTCGCTTATTTAGAAGTGGCGCGCCCTCTCGAGGGCG), respectively. The Rnt1p substrates were amplified for insertion into pCS1749 by gap‐repair using the forward and reverse primers mCherry_gap_fwd_prmr (5′‐GGTGGCATGGATGAACTATACAAATAATAAAGCCTAGGTCTAGAGGCG‐3′) and mCherry_gap_rev_prmr (5′‐TGACATAACTAATTACATGATGCGGCCCTCCCCTCTCGAGGGCG‐3′). In the case of digestion and ligation, the PCR products were digested with the unique restriction sites AvrII and XhoI, which are located 3 nts downstream of the yEGFP3 or ERG9 stop codon and upstream of the ADH1 terminator. Following construction and sequence verification of the desired vectors, 100–500 ng of each plasmid was transformed into W303. In the case of gap‐repair (for pCS321 and pCS1749), 250–500 ng of the PCR product and 100 ng of pCS321 digested with AvrII and XhoI were transformed into the yeast strain. All yeast strains harboring cloned plasmids were maintained on synthetic complete media with an uracil dropout solution containing 2% dextrose at 30°C.
3′ UTR replacement cassette and integration
The ERG9‐RNT1 replacement cassettes were synthesized through PCR amplification from the appropriate pCS1813‐based plasmids using forward and reverse primers ERG9‐1150_fwd_prmr (5′‐AATTACCTCCTAACGTGAAGCCAAATGAAACTCCAATTTTCTTGAAAGTT‐3′) and Rnt1p_cassette_rev_prmr2 (5′‐GGCCTCTACCTATTATGTAAGTACTTAGTTATTGTTCGGAGTTGTTTGTTAATACGACTCACTATAGGGAGACCGGCAGA‐3′), respectively. These PCR products extend from 1150 nts in the ERG9 gene to the end of the second loxP site, with an overhang extension comprising 50 nts of homology to the native ERG9 3′ UTR. Each integration cassette (approximately 1–5 μg) was transformed into yeast, as previously described. The integrants were selected and maintained on YPD plates with 200 mg/ml G418.
Rnt1p substrate characterization assays
S. cerevisiae cells harboring pCS321‐based and pCS1749‐based plasmids were grown on synthetic complete media with an uracil dropout solution and the appropriate sugars (2% raffinose and 1% sucrose for pCS321; 2% dextrose for pCS1749) overnight at 30°C. The cells were back‐diluted the following morning into fresh media (4.5 ml total volume in test tubes and 450 μl in deep‐well plates) to an optical density at 600 nm (OD600) of 0.1 and grown again at 30°C. For pCS321‐based plasmids, after 1 h, 0.5 ml (test tubes) or 50 μl (plates) of 20% galactose (2% final concentration) or water (non‐induced control) was added to the cell cultures. The cells were grown for another 4.5 h before measuring the fluorescence levels or collecting cells for RNA extraction.
S. cerevisiae integrated with Rnt1p hairpins or its controls were grown on YPD overnight at 30°C. The cells were back‐diluted the following morning into fresh media (5 ml total volume in test tubes) and grown again for 3 h at 30°C. After 3 h, the cells were back‐diluted to an OD600 of 0.1 (for RNA extraction and growth rate determination, 5 ml total volume) or 0.05 (for ergosterol quantification, 7 ml total volume) and grown for an appropriate length of time depending on the application.
On a SAFIRE plate reader (TECAN, Männedorf, Switzerland), GFP fluorescence was read from 200 μl of cells with an excitation wavelength of 485 nm, an emission wavelength of 515 nm and a gain of 100. The population‐averaged fluorescence readings were normalized to the amount of cells by dividing the relative fluorescence units by the OD600 of the sample. On the Quanta flow cytometer (Beckman Coulter, Fullerton, CA), the distribution of GFP fluorescence was measured with the following settings: 488‐nm laser line, 525‐nm bandpass filter and photomultiplier tube setting of 5.83. Data were collected under low flow rates until 10 000 viable cell counts were collected. A non‐induced cell population was used to set a gate to represent GFP‐negative and GFP‐positive populations. The median fluorescence of the positive population was measured from three identically grown samples. The LSRII flow cytometer (Becton Dickinson Immunocytometry Systems) was used to measure ymCherry fluorescence from p1749‐based plasmids. ymCherry was excited at 532 nm and measured with a splitter of 600 nm LP and a bandpass filter of 610/20 nm. A DAPI stain (excited at 405 nm and measured with a bandpass filter of 450/50 nm) was used to gate for cell viability. The mean fluorescence was measured from three identically grown samples and baseline subtracted with an empty vector control. Reported values and their error are calculated from the mean and standard deviation from the triplicate data, respectively.
Quantification of cellular transcript levels
Total RNA from S. cerevisiae was collected by a standard hot acid phenol extraction method (Caponigro et al, 1993) and followed by DNase I (New England Biolabs) treatment to remove residual plasmid DNA according to manufacturer's instructions. cDNA was synthesized from 5 μg of total RNA with gene‐specific primers for yEGFP3, ymCherry, ERG9 and ACT1 (Ng and Abelson, 1980) (rnt1p_rtpcr_rev2 and ACT1_rtpcr_rev) and SuperScript III Reverse Transcriptase (Invitrogen) according to manufacturer's instructions. The forward and reverse primers for yEGFP3 quantification are rnt1p_rtpcr_fwd2 (5′‐CGGTGAAGGTGAAGGTGATGCTACT‐3′) and rnt1p_rtpcr_rev2 (5′‐GCTCTGGTCTTGTAGTTACCGTCATCTTTG‐3′), respectively; for ymCherry quantification the primers used are mCherry_qrtpcr_fwd (5′‐AAGGGTTTAAGTGGGAGCGTGTGA‐3′) and mCherry_qrtpcr_rev (5′‐AAGGCACCATCTTCAGGGTACATTCG‐3′), respectively; for ERG9 quantification the primers used are erg9_rtpcr_fwd (5′‐AACTGTTGAACTTGACCTCCAGATCGTTTG‐3′) and erg9_rtpcr_rev (5′‐GGCTCTGTCCTTCACATCGGGGGCATTTCC‐3′), respectively; for ACT1 quantification the primers used are ACT1_rtpcr_fwd (5′‐GGCATCATACCTTCTACAACGAAT‐3′) and ACT1_rtpcr_rev (5′‐GGAATCCAAAACAATACCAGTAGTTCTA‐3′), respectively. Relative transcript levels and their error were quantified in triplicate from three identical reactions from the cDNA samples by using an appropriate primer set and iQ SYBR Green Supermix (Bio‐Rad, Hercules, CA) on an iCycler iQ qRT–PCR machine (Bio‐Rad) according to the manufacturer's instructions. For each run, a standard curve was generated for yEGFP3, ymCherry or ERG9 and a housekeeping gene, ACT1, using a dilution series for a control representing no insertion of an Rnt1p substrate. Relative transcript levels were first individually determined for each sample and then the values for yEGFP3, ymCherry and ERG9 were normalized by their corresponding ACT1 values.
Cell growth rate determination
At multiple time points during a course of 7 h, 200 μl were taken from a yeast culture and the OD600 measured on a SAFIRE plate reader. The growth rate, k, and its standard error were analyzed using Prism 5 (GraphPad), by fitting the data to an exponential growth curve.
Cellular ergosterol quantification
The method for quantification of cellular ergosterol levels was adapted from previously developed protocols (Arthington‐Skaggs et al, 1999; Asadollahi et al, 2008). Briefly, yeast cells were harvested after 8 h, with the OD600 recorded and collected by centrifugation at 3000 r.p.m. for 5 min. The cells were washed with water and centrifuged again. A volume of 10 ml of 25% alcoholic KOH (25% KOH, 60% (v/v) ethanol) was added to the cell pellet and vortexed. The suspension was transferred to a 50 ml Falcon tube and saponified by incubating at 90°C for 3 h. After cooling to room temperature, the non‐saponified sterols were extracted by adding 5 ml of heptane, vortexing and collecting the heptane layer once it had clarified. The heptane layer was directly applied to a 96‐well UV plate (Greiner Bio‐One) and its absorbance was read in the UV spectrum on a SAFIRE plate reader. A REV was calculated from the following equation:
The reported value and error were determined from the mean and standard deviation, respectively, from three identical aliquots from sterol extractions.
In vitro transcription of Rnt1p substrates
All Rnt1p substrates were PCR amplified to include an upstream T7 promoter site and A‐rich sequences flanking the hairpin using forward and reverse primers Rnt1p‐T7‐PCR_fwd_prmr (5′‐TTCTAATACGACTCACTATAGGGACCTAGGAAACAAACAAAGTTGGGC‐3′) and Rnt1p‐T7‐PCR_rev_prmr (5′‐CTCGAGTTTTTATTTTTCTTTTTGCCGGGCG‐3′), respectively. A measure of 1–2 μg of PCR product was transcribed with T7 Polymerase (New England Biolabs) in the presence and absence of α‐P32‐GTP. The 25‐μl reaction consisted of the following components: 1 × RNA Pol Reaction Buffer (New England Biolabs), 3 mM rATP, 3 mM rCTP, 3 mM rUTP, 0.3 mM rGFP, 1 μl RNaseOUT (Invitrogen), 10 mM MgCl2, 2 mM DTT, 1 μl T7 Polymerase and 0.5 μCi α‐P32‐GTP. Unincorporated nucleotides were removed from the reactions by running the samples through NucAway Spin Columns (Ambion, Austin, TX) according to the manufacturer's instructions.
In vitro Rnt1p substrate cleavage assay
Cleavage assays were performed on Rnt1p substrates as previously described (Lamontagne and Elela, 2001; Lamontagne and Elela, 2004). Briefly, a 10‐μl mixture of RNA and Rnt1p (see Supplementary Methods) was incubated at 30°C for 15 min in Rnt1p reaction buffer (30 mM Tris (pH 7.5), 150 mM KCl, 5 mM spermidine, 20 mM MgCl2, 0.1 mM DTT and 0.1 mM EDTA (pH 7.5)). RNA concentrations were varied from 0.1 to 1.0 μM and the Rnt1p concentration was 2.3 μM. The cleavage reaction products were separated on an 8% denaturing polyacrylamide gel run at 35 W for 30 min. Gels were transferred to filter paper and analyzed for relative substrate and product levels through phosphorimaging analysis on a FX Molecular Imager (Bio‐Rad). The levels of cleaved RNA product were determined and fit to a Michaelis–Menten model using Prism 5, in which a relative Vmax was calculated and reported with the standard error determined by the fit of the model.
In vitro Rnt1p substrate mobility shift assay
Mobility shift assays were performed as previously described (Lamontagne and Elela, 2001; Lamontagne and Elela, 2004). Briefly, a 10‐μl mixture of RNA and Rnt1p were incubated on ice for 10 min in Rnt1p‐binding buffer (20% (v/v) glycerol, 30 mM Tris (pH 7.5), 150 mM KCl, 5 mM spermidine, 0.1 mM DTT and 0.1 mM EDTA (pH 7.5)). The RNA concentration in all samples was 200 nM and the Rnt1p concentration ranged from 0 to 1.7 μM. The binding reaction products were separated on a 6% native polyacrylamide gel run at 350 V until the samples entered the gel and then at 150 V for 2 h. Gels were transferred to filter paper and analyzed for free RNA and RNA–Rnt1p complex levels through phosphorimaging analysis on a FX Molecular Imager. The fraction of unbound RNA to total RNA was determined and fit to a modified Scatchard model using Prism 5, in which KD value was calculated and reported with the standard error determined by the fit of the model.
We thank K Hoff, S Bastian and FH Arnold for assistance in the purification of Rnt1p and J Liang for assistance with in vitro assays. This work was supported by the National Science Foundation (CAREER award to CDS; CBET‐0917705) and the Alfred P Sloan Foundation (fellowship to CDS).
Author contributions: AHB designed research, performed research and wrote the paper; CDS designed research and wrote the paper.
Conflict of Interest
The authors declare that they have no conflict of interest.
Supplementary Figures S1–5, Supplementary Tables SI and SII [msb20114-sup-0001.pdf]
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