Competence is a physiological state, distinct from sporulation and vegetative growth, that enables cells to bind and internalize transforming DNA. The transcriptional regulator ComK drives the development of competence in Bacillus subtilis. ComK is directly required for its own transcription as well as for the transcription of the genes that encode DNA transport proteins. When ComK is sequestered by binding to a complex of the proteins MecA and ClpC, the positive feedback loop leading to ComK synthesis is interrupted. The small protein ComS, produced as a result of signaling by a quorum‐sensing two‐component regulatory pathway, triggers the release of ComK from the complex, enabling comK transcription to occur. We show here, based on in vivo and in vitro experiments, that ComK accumulation is also regulated by proteolysis and that binding to MecA targets ComK for degradation by the ClpP protease in association with ClpC. The release of ComK from binding by MecA and ClpC, which occurs when ComS is synthesized, protects ComK from proteolysis. Following this release, the rates of MecA and ComS degradation by ClpCP are increased in our in vitro system. In this novel system, MecA serves to recruit ComK to the ClpCP protease and connects ComK degradation to the quorum‐sensing signal‐transduction pathway, thereby regulating a key developmental process. This is the first regulated degradation system in which a specific targeting molecule serves such a function.
Competence is defined as the ability to bind and internalize exogenous DNA. In Bacillus subtilis, competence is a physiological state, distinct from vegetative growth and sporulation, which occurs at the onset of late exponential growth under specific nutritional conditions. Competence is expressed in a distinct subpopulation of non‐dividing cells which are arrested for DNA replication and the synthesis of stable RNA (B.J.Haijema, M.Albano, J.Hahn and D.Dubnau, unpublished; Nester and Stocker, 1963; McCarthy and Nester, 1967; Hadden and Nester, 1968; Haseltine‐Cahn and Fox, 1968; Dooley et al., 1971). When resuspended in fresh growth medium, competent cells can escape this arrested state, but only after a pronounced lag (Nester and Stocker, 1963).
A set of proteins encoded by late competence genes are responsible for the binding, processing and internalization of transforming DNA, and are present only in the competent subpopulation (reviewed in Dubnau, 1997). The transcription factor ComK is needed for the transcription of these genes, and is active and expressed only in the cells fated to become competent (van Sinderen et al., 1995; Hahn et al., 1996). ComK also activates the transcription of genes needed for recombination and DNA repair (Haijema et al., 1996). ComK synthesis and activity is a crucial checkpoint, committing a cell to competence development. ComK control is embedded in a complex signal transduction network, linking it to other developmental processes in Bacillus such as sporulation, and the synthesis of degradative enzymes and secondary metabolites (Dubnau, 1993; Grossman, 1995).
Since ComK is the factor that drives a cell to competence, and since competence is accompanied by the blockage of essential cell functions, it is no surprise that ComK synthesis is subject to a number of finely tuned and redundant regulatory circuits. The transcription of comK is activated by ComK itself and is controlled further by additional transcriptional regulators (Dubnau, 1993; Grossman, 1995). In addition, ComK is activated and overproduced in strains carrying null mutations in mecA or clpC (formerly known as mecB) (Dubnau and Roggiani, 1990; Roggiani et al., 1990). MecA normally binds to ComK and inhibits its activity, while ClpC enhances this inhibition by binding to MecA. When sequestered in a ternary complex with MecA and ClpC, ComK is not able to activate its own transcription (Kong and Dubnau, 1994; Turgay et al., 1997). Mutational inactivation of either mecA or clpC releases ComK and permits its overproduction.
ComS, a small protein of 46 amino acid residues, is necessary for competence development (D'Souza et al., 1994; Hamoen et al., 1995) and is expressed in response to the quorum‐sensing pheromones ComX and CSF (Magnuson et al., 1994; Solomon et al., 1996; Lazazzera et al., 1997). ComS induces the increased synthesis of ComK by dissociating its ternary complex with ClpC and MecA, thereby releasing active ComK which in turn activates its own transcription. Thus, ClpC, MecA and ComK, together with the signaling protein ComS, form a regulatory device controlling the activity of ComK. In cells where ComS releases ComK, a burst of ComK expression occurs, because of the positive autoregulation of comK expression by ComK (Hahn et al., 1996; Turgay et al., 1997). For unknown reasons, this release occurs only in the subpopulation of cells fated for competence.
MecB was shown to be a heat shock protein and was identified from its sequence as ClpC (Krüger et al., 1994; Msadek et al., 1994). ClpC is a member of the Hsp100 family of proteins which includes ClpA, ClpB, ClpX and ClpY in Escherichia coli, and Hsp104 in Saccharomyces cerevisiae (Schirmer et al., 1996). In E.coli, ClpA and ClpX independently interact with the protease ClpP to form high molecular weight oligomers which can degrade specific substrate proteins (Gottesman et al., 1997). Both ClpA and ClpX are ATPases which possess chaperone‐like activity and are responsible for the substrate specificity of the ClpAP and ClpXP proteases (Wickner et al., 1994; Wawrzynow et al., 1995). ClpC, ClpE, ClpX and ClpY (CodW), but not ClpA or ClpB homologs, were identified as members of the Hsp100 family in the B.subtilis genome (Kunst et al., 1997).
The mutational inactivation of mecA or clpC results in ComK overexpression in all of the cells in a culture grown to stationary phase and the appearance of cells which exhibit defects in nucleoid segregation and reduced viability (Hahn et al., 1995). It was proposed that ComK was responsible for the arrest in DNA replication which normally accompanies competence development and that MecA is essential for the escape from competence. Our earlier work led us to postulate that cells escape from the competent state simply by the binding of ComK in a complex with MecA and ClpC. However, as reported in this paper, ComK is present in the competent cells in vast excess over the levels of MecA and ClpC. This observation, and the importance of ComK for competence development, led us to examine the in vivo stability of ComK. We show that ComK is rapidly degraded during the escape from competence, and that this degradation requires MecA, ClpC and ClpP. Using purified proteins we demonstrate in vitro that the ATP‐dependent degradation of ComK by ClpCP requires MecA and occurs more rapidly in the absence of ComS. Furthermore, when ComS is present, MecA is degraded by ClpCP, as is ComS itself. These observations suggest that the regulation of competence development as well as the escape from competence, rely on regulated proteolysis. In this novel system, MecA serves to recruit ComK to the ClpCP protease, and connects ComK degradation to the quorum‐sensing signal‐transduction pathway, thereby regulating a key developmental process.
In vivo amounts of ComK, MecA and ClpC
Since MecA and ClpC are involved in the control of ComK synthesis and activity, we measured the cellular content of these three proteins by quantitative Western blotting, reasoning that such data would provide insight into the in vivo operation of this system. It became clear that ComK is present in considerable excess over MecA and ClpC, thus excluding models for the escape from competence simply by the rebinding of ComK to MecA/ClpC.
These measurements are shown in Table I. The number of MecA molecules/cell was constant, whereas ClpC doubled in amount from t0 to t2. ComK was not detectable at t0, and then increased dramatically, consistent with its positive autoregulation. MecA (Kong and Dubnau, 1994), and presumably ClpC, were expressed in all of the cells in a competent culture. ComK, on the other hand, was only expressed in ∼5‐10% of the cells and a 10‐fold correction was therefore applied in Table I. ComK was undetectable at t0, and there were 12 000 molecules per competent cell at t1 and 91 000 at t2. Since ComK binds to DNA as a tetramer (Hamoen et al., 1998), the number of active ComK entities per cell at t2 could be estimated as 22 750. The high content of ComK at t2 is unusual for a regulatory protein, and is roughly comparable to the content of HU molecules (30 000 or 15 000 dimers) in the smaller cells of E.coli (Pettijohn, 1996). MecA is present in solution as a dimer (M.Persuh and D.Dubnau, unpublished), leading to an estimate of 700 entities/cell at t2. We postulate that ClpC is present in a complex similar to those containing ClpA or ClpX in E.coli and that there are therefore either 6 or 12 ClpC monomers per complex, since ClpAP and ClpXP structures have been observed containing either single or double rings of the ATPase subunits (Kessel et al., 1995; Grimaud et al., 1998). This leads to an estimate of 650‐1300 active units of ClpC per cell, comparable to the estimate for MecA. This excess of ComK over MecA and ClpC led us to consider a model for the regulation of competence which involves the degradation of ComK.
ComK is degraded during the escape from competence
The data presented below demonstrate that ComK is rapidly degraded during the escape from competence and that MecA and ClpC are needed for this degradation. Cells were grown to t2 in competence medium and then diluted 1:20 into fresh competence medium containing rifampicin and tetracycline. This regime permitted the reversal of competence‐inducing signals and prevented the synthesis of new ComK. Samples were taken at intervals after dilution, and extracts were analyzed by Western blotting for the presence of ComK. ComK was degraded at a constant rate and was eliminated after ∼2 h (Figure 1). In mecA and clpC backgrounds on the other hand, ComK was stable. In a strain carrying a multicopy comS plasmid the decay rate of ComK was decreased ∼2‐fold. Since ComK was stabilized in strains lacking MecA and ClpC, we propose that these proteins are part of the degradative machinery. In contrast, since ComK degradation was slowed in a strain which overexpresses ComS, this protein must be involved in a mechanism which decreases the rate of ComK degradation. The experiment shown in Figure 1 was performed in the presence of antibiotics to block transcription and translation. Similar results were obtained in the absence of these antibiotics, suggesting that little additional ComK synthesis was occurring.
Neither ClpC nor MecA are known to have proteolytic activities, nor were they able to degrade ComK in vitro (Turgay et al., 1997). One obvious candidate for a proteolytic component involved in the degradation of ComK is ClpP, since this protein is known to form proteasome‐like complexes with ClpA or ClpX in E.coli. It seemed likely from these results that binding to MecA and ClpC targets ComK for degradation by ClpP. We therefore decided to test the effect of a clpP mutation on competence.
MecA accumulates in clpP and clpC mutants
To determine the effect of a clpP mutation on the stability of ComK, we insertionally inactivated the clpP gene of B.subtilis and tested the resulting strain for its ability to become competent and express comK‐lacZ. Unexpectedly, the clpP mutant strain was not competent, exhibited no comK‐lacZ activity and had no detectable ComK, as determined by Western blotting. Transcription of comK‐lacZ could be partially restored in the clpP mutant strain by the introduction of a mecA mutation (data not shown). Similar results have recently been reported independently (Msadek et al., 1998). These observations can be explained if ClpP is responsible for the degradation of MecA, since ComK acts as an activator of its own transcription, and an excess of MecA prevents this positive autoregulatory activity by binding ComK (Kong and Dubnau, 1994). To determine whether MecA accumulates in a clpP strain, extracts of cells grown to t2 in competence medium were analyzed by Western blotting. Excess MecA accumulated in the clpP strain, as well as in the clpC strain (Figure 2). In contrast, a null mutation in clpX had no effect. Mutations in other ATPase/protease loci [codW (clpY), codX (clpQ)] and lonA also had no detectable effects on the level of MecA (not shown). These results suggest that ClpC and ClpP act to degrade MecA, possibly acting together in a ClpC‐ClpP complex. Although inactivation of either clpP or clpC results in the accumulation of excess MecA, only the clpP mutation results in a failure to synthesize ComK. This is presumably because in the absence of ClpC the affinity of MecA for ComK is lowered (Turgay et al., 1997), and the accumulated MecA therefore has little or no effect on the level of active ComK in the cell.
ClpP, ClpC and MecA are required in vivo for the degradation of ComK
Using a genetic background that avoids the inhibitory effect of MecA on ComK synthesis, we demonstrated that ClpP, as well as MecA and ClpC, were necessary for the in vivo degradation of ComK. To accomplish this, we used a strain carrying a Tn10 insertion in the ComK‐Box of the comK promoter (Luttinger et al., 1996; Hamoen et al., 1998), thereby completely disrupting transcription from the comK locus. An additional copy of the comK gene under the control of the inducible Pxyl promoter was placed in the chromosomal amyE locus. In this strain (BD2524), the expression of ComK is dependent on the presence of xylose but not on the PcomK promoter (Hahn et al., 1996). BD2524 was grown in competence medium with xylose induction, and at hourly intervals samples were taken and processed for Western blot analysis. In this background, ComK was detected at t0 and at t1 but was barely detectable after t1 (Figure 3). This is different from the wild‐type situation in which the amount of ComK peaks at t2 under the same conditions (not shown). ComK disappears earlier in the Pxyl strain background because the Pxyl promoter is weak and the net synthesis of ComK is low in this background (Hahn et al., 1996). Mutations in clpP, mecA and clpC were introduced into the same xylose‐inducible background and these strains were similarly grown and analyzed (Figure 3). The amount of ComK in these mutant strains was markedly increased and ComK was stabilized, consistent with the experiment presented in Figure 1. Mutations in codW (clpY), codX (clpQ) and lonA did not stabilize ComK in similar experiments (data not shown). We conclude that ClpP, as well as MecA and ClpC, is required for ComK degradation.
ClpCP degrades ComK, MecA and ComS in vitro
Using purified proteins, we next demonstrated in vitro that the ATP‐dependent degradation of ComK requires MecA, ClpP and ClpC and is decreased in rate in the presence of ComS. To obtain ClpP protein we fused the B.subtilis clpP to a sequence encoding a C‐terminal His6 tag, and expressed this construct in E.coli for purification. When this fusion construct was integrated into the B.subtilis chromosome, it was able to substitute for a wild‐type clpP gene for competence development (not shown).
We first tested the in vitro stability of ComK and the dependence of this stability on ClpP, MecA, ClpC, ComS and ATP. Degradation of ComK occurs in vitro, and is dependent on the presence of ATP (Figure 4, compare lane 2 with lane 6), ClpC (Figure 4, lanes 5 and 9), ClpP (Figure 4, lanes 5 and 8) and MecA (Figure 4, lanes 5 and 7). The presence of ComS reduces the rate of degradation of ComK significantly (Figure 4, compare lanes 3 and 4 with 5 and 6). These results reflect those obtained in vivo with appropriate mutants.
Figure 5A and B presents an experiment testing the in vitro stabilites of MecA and ComS, and the dependence of their stabilities on ClpP, ComS and ATP. MecA is degraded if ComS, ClpP and ATP are present, but is stable if ClpP or ATP are absent. Figure 5B shows that the degradation of MecA is stimulated in the presence of ComS, although MecA is still degraded when ComS is omitted. The in vivo stabilities of ComK and MecA are therefore affected in opposite ways by the presence of ComS. In an independent experiment (not shown) we have found that when ClpC is omitted, MecA is also not degraded, as expected from the dependence of its degradation on ATP. In additional experiments in which pyruvate kinase and phosphoenolpyruvate were omitted, we have observed that ADP cannot replace ATP for the degradation of either ComK or MecA (not shown).
It is also apparent that ComS is degraded along with MecA (Figure 5A). This degradation requires MecA and ClpC (not shown), as well as ClpP and ATP. In several experiments we have noted that the rate of ComS degradation is greater than that of MecA (not shown).
Interaction of ClpP with ClpC
We reported previously that the addition of MecA to ClpC stimulates the ATPase activity of ClpC and that the addition of ComS causes a further stimulation, but only when MecA is present (Turgay et al., 1997). ClpC by itself exhibits very low ATPase activity. We have examined the effect of ClpP on the ClpC ATPase in various combinations with ComS and MecA (Figure 6). When ClpP alone was added to ClpC, little ATPase activity was observed. However, when ClpP was added to ClpC in the presence of MecA or of MecA plus ComS, a further increase was observed over that observed when these components were added in the absence of ClpP. This is comparable to the results obtained with ClpAP in E.coli, as discussed below.
To explore further the interaction of ClpP and ClpC, we used immunoaffinity chromatography with ClpP columns to demonstrate the ATP‐dependent interaction of ClpP with ClpC. This experiment demonstrated an interaction (not shown). Under the conditions of the experiment the binding of ClpP to ClpC was apparently not strong, but was ATP‐dependent. It is likely that when attached to the column material ClpP cannot form an appropriate ring structure (Wang et al., 1997), and that its binding to ClpC is therefore suboptimal. In vitro we have observed ATP‐dependent proteolysis of MecA, ComS and ComK, dependent on the presence of ClpC and ClpP (Figures 4 and 5). We conclude that ClpC and ClpP must interact to form a proteolytic complex analogous to that formed by ClpA‐ClpX and ClpP in E.coli.
A decisive step leading to competence development in B.subtilis is the control of the activity, and consequently the synthesis, of the positively autoregulated competence transcription factor ComK. In this study we show that as a result of the explosive synthesis of ComK which begins at t0, the number of ComK tetramers increases to ∼20 000 per cell, which can be compared to the content of the histone‐like HU protein in E.coli. ComK has a high binding affinity for certain sites on DNA, and as is true of other DNA‐binding proteins, also exhibits non‐specific DNA binding (Hamoen et al., 1998). We have observed by immunofluorescence with anti‐ComK antibodies that ComK is associated with the nucleoid of competent cells (B.J.Haijema and D.Dubnau, unpublished). ComK is known to bend DNA in the vicinity of its binding sites (Hamoen et al., 1998). The large number of ComK molecules bound to the nucleoid DNA (potentially ∼1 tetramer per 200 base pairs) may not only cause the activation of transcription of the late competence, recombination and DNA repair genes, but may also have structural consequences for the chromosomes, and ComK binding might directly cause the replication arrest and transcriptional silencing characteristic of competent cells (Nester and Stocker, 1963; McCarthy and Nester, 1967).
Proteolysis during the escape from competence
Clearly, the synthesis of ComK has a profound effect on the physiology of competent cells and these cells can only escape competence when MecA is present (Hahn et al., 1995). The present study demonstrates that ComK is degraded as the cells escape from competence and that MecA is needed to target ComK for this degradation. We have demonstrated in vitro that the degradation of ComK is accomplished by ClpC and ClpP, and that this degradation is ATP‐dependent. ComS, which is known to cause the release of ComK from binding to ClpC and MecA (Turgay et al., 1997), protects ComK from degradation both in vivo and in vitro. In addition, MecA and ComS are themselves degraded by ClpCP in vitro, in the presence of ATP. ComK degradation is needed for the escape from competence to rid the cell of this toxic molecule. However, it is probable that an interruption of ComK synthesis plays a part in this escape. It was noted above that ComK degradation was also observed in the absence of added antibiotics, suggesting that ComK synthesis was essentially turned off. This inference is supported by experiments using an in‐frame comK‐lacZ fusion, which demonstrated a marked decrease in the rate of comK expression at ∼t2 (Hahn et al., 1994). Once the rate of ComK degradation exceeds its rate of synthesis, the ComK level in the cell will continue to decline, because ComK is needed for its own transcription.
Proteolysis during the development of competence
These observations add an important new dimension to the regulation of competence (Figure 7). During exponential growth, any ComK synthesized is bound by MecA and ClpC, and is thereby prevented from acting as a transcription factor (Turgay et al., 1997). The present results suggest that the bound ComK is also targeted for degradation by ClpCP. In the Pxyl‐comK clpP strain, a clear ComK signal appears before t0, whereas in the clpP+ strain a signal is not apparent until t0 (Figure 3). In addition, we have observed that in the Pxyl‐comK background, the mutational inactivation of clpP raises the expression of a lacZ fusion to the late competence gene comG during exponential growth (not shown). Since comG transcription is ComK‐dependent, this experiment shows that the clpP strain contains an excess of functional ComK. These observations demonstrate that in this background, in the clpP mutant, an increased amount of ComK is present before t0. This implies that the degradative pathway is normally needed to fully restrain the synthesis of ComK prior to t0.
At, or just before t0, the quorum‐sensing pathway induces the synthesis of ComS (D'Souza et al., 1994; Hamoen et al., 1995). The presence of ComS prevents the binding of ComK to complexes containing MecA and ClpC. ComS appears to act by binding directly to MecA (Liu et al., 1996; Turgay et al., 1997) presumably altering the conformation of MecA and reducing its affinity for ComK. When ComS binds to MecA, the rate of ComK degradation by ClpCP decreases, because ComK is released (Figure 7). More ComK is now available to activate the transcription of its own gene, resulting in the synthesis of additional active ComK, until it is present in excess over MecA and ClpC. At this point the synthesis of ComK is free to increase dramatically. In this mechanism the degradation rate of ComK is adjusted in accordance with the input concentration of ComS, but ComK synthesis is regulated in a switch‐like fashion, due largely to the positive autoregulation of ComK transcription.
Although ClpCP is needed to prevent the accumulation of excess MecA (Figure 2), it is not certain that the enhanced degradation of MecA in the presence of ComS is a regulatory process. On the other hand, the failure of the clpP strain to synthesize ComK suggests that the proteolytic role of ClpCP is necessary to restrain the accumulation of MecA. An alternative explanation, that in a clpP mutant, mecA transcription is somehow increased, was refuted by the use of a mecA‐lacZ transcriptional fusion (Msadek et al., 1998).
The in vitro degradation of ComS by ClpC and ClpP in the presence of MecA and ATP is highly suggestive. We believe that this represents a timing mechanism, placing a limit on the synthesis of ComK. The degradation of ComS would ensure that as the level of active ComK increases, ComS decreases in parallel. As the ComS level decreases, ComK will be increasingly rebound by ClpCP‐MecA and targeted for degradation (Figure 7). We have obtained independent evidence that ComS is quite unstable in vivo (not shown), consistent with this hypothesis and with our in vitro data.
Protein‐protein interactions and substrate recognition during proteolysis
Since the addition of ClpP to ClpC does not stimulate its very low intrinsic ATPase activity (Figure 6B), the interaction of the ATPase subunit with ClpP is clearly different from its interaction with potential substrates. This can be compared to the result obtained in the E.coli system; when ClpP was added to ClpA, a decrease in ATPase activity was noted (Hwang et al., 1988). In the presence of ClpP, the behavior of the ClpC ATPase upon the addition of MecA alone or in combination with ComS was similar to that described previously in the absence of ClpP (Turgay et al., 1997), except that a further stimulation was observed. When ClpP is absent, the stimulation of ClpC ATPase upon addition of potential substrates probably reflects the chaperone‐like activity of ClpC. In the absence of ClpP this activity is in a sense futile, since it does not lead to degradation. The additional stimulation noted when ClpP was present may therefore reflect the coupling of ATP hydrolysis to degradation. The stimulation of the ATPase activity of ClpCP when MecA alone or in combination with ComS was added is comparable to the published results obtained with ClpP and ClpA when the substrate casein was present (Hwang et al., 1988).
The B.subtilis ClpP not only associates with ClpC, but also with ClpX (Y.‐I.Kim and T.Baker, personal communication) and possibly with ClpE (Msadek et al., 1998). This may explain the wide range of phenotypes of a clpP strain, affecting heat shock, motility, degradative enzyme synthesis, sporulation and competence (Gerth et al., 1998; Msadek et al., 1998).
MecA recognizes ClpC, ComK and ComS, and is needed to target the latter two proteins for degradation by ClpCP. MecA therefore diverts the proteolytic activity of ClpCP from its role in general stress management functions, such as degrading aggregated or misfolded proteins, to a regulatory function in competence development. MecA connects signaling via the quorum‐sensing system with the regulation of ComK degradation. In this way the quorum‐sensing two‐component signal‐transduction cascade modulates the stability of a key regulatory protein. It has been shown in vivo in E.coli that the degradation of σs by ClpXP is influenced by the response regulator SprE (RssB). SprE modulates the degradative activity of ClpXP toward σs in response to environmental signals (Zhou and Gottesman, 1998).
The use of a specific targeting molecule such as MecA seems to be unique at least among the prokaryotic proteolysis systems. In E.coli, a wide variety of substrate proteins for the ATP‐dependent proteases ClpAP, ClpXP, Lon and FtsH have been identified (Gottesman et al., 1997). It has been demonstrated that some of these have C‐terminal sequences which can be recognized directly by ClpA or ClpX (Levchenko et al., 1997) and the ‘N‐end rule’ has also been implicated in targeting degradation by ClpAP (Tobias et al., 1991). Substrate recognition by the eukaryotic proteasome occurs by a variety of mechanisms. The eukaryotic 26S proteasome is built from multiple protein subunits, including a 19S regulatory complex, which contain components necessary for protein substrate binding. Some of these components may play a role in targeting analogous to that of MecA and ClpC. The main substrate proteins for the proteasome are targeted for degradation by ubiquitination although other recognition mechanisms exist (Coux et al., 1996).
Proteolysis and bacterial development
The competence system is one of three bacterial systems in which degradation of a key regulatory molecule is known to control a developmental program. In each case development yields two distinct cell types. During sporulation in B.subtilis, the mother‐cell‐specific sigma factor σE is degraded in the forespore compartment, and a regulatory phosphatase, required for the activation of the forespore sigma factor σF is preferentially degraded in the mother‐cell compartment (Pogliano et al., 1997). The proteases involved in these processes have not been identified and the nature of the regulatory mechanism has not been elucidated, although compartment‐specific proteolysis requires the chromosome transport protein SpoIIIE. The activity of the transcriptionally active response regulator CtrA, which inhibits DNA replication in Caulobacter crescentus, is regulated by phosphorylation and the level of CtrA in the cell is regulated by proteolysis (Domian et al., 1997; Quon et al., 1998). In this case ClpXP is responsible for the proteolysis, which occurs in the portion of the pre‐divisional cell destined to give rise to the stalked daughter. Although the nature of the mechanism that controls proteolysis is not yet known, additional factors that regulate proteolysis were postulated (Domian et al., 1997). Such molecules might have roles analogous to those of MecA or ComS.
Competence also involves the generation of two cell types, characterized by the differential synthesis and accumulation of a regulatory molecule. Since overproduction of ComK (in a mecA strain) causes all the cells in a population to follow the competence developmental pathway, it is logical to infer that the synthesis of ComK is a competence‐determining event. As in the cases of sporulation and Caulobacter development, the crucial molecule is expressed in a cell‐type‐specific manner. Overexpression of ComS results in a shift of the developmental program in the direction of competence in most cells in a population (M.Albano and D.Dubnau, unpublished; Hahn et al., 1996; Liu et al., 1996). Differential transcription of comS is not the basis of this developmental decision, since its promoter is transcribed in all the cells of a pre‐competent population (Hahn et al., 1994). It is possible that differential degradation of ComS may be a cell‐type‐determining event in competence development.
Materials and methods
DNA manipulations and standard molecular biological methods were as described in Sambrook et al. (1989). Growth of B.subtilis in competence medium, transformation and β‐galactosidase activity measurements were as previously described (Albano et al., 1987). The strains used are described in Table II. Electrophoresis was carried out with standard SDS‐polyacrylamide (Laemmli, 1970) or Tricine gels (Schagger and von Jagow, 1987). Protein was determined with Bio‐Rad reagents (Bradford, 1976). Western blotting was as described previously (Kong and Dubnau, 1994). For signal quantitation, films were scanned and analyzed densitometrically.
Strain construction and protein purification
An internal clpP fragment was amplified by PCR from chromosomal IS75 DNA using the primers P1 (5′‐CGG AAT TCA TTG AAC AAA CGA AAC CGC‐3′) and P2 (5′‐CGG AAT TCT TCA CTG TTT GGA AGC GC‐3′). This fragment was cloned into the EcoRI site of pUCCM18 (Inamine and Dubnau, 1995), resulting in pED221. The clpP strain BD2590 was constructed by transforming pED221 into IS75, leading to a disruption of clpP.
clpP was amplified by PCR from chromosomal IS75 DNA using the primers QClpPSph (5′‐GGA GGC AGC ATG CAA TTA ATA CCT ACA GTC‐3′) and QClpPBam (5′‐GTG GGA TCC CTT TTT GTC TTC TGT GTG AG‐3′). The product was cloned into pQE70 (Qiagen) using the underlined BamHI and SphI sites. The structure of the resulting plasmid (pclpP11) was verified by sequencing. pClpP11 was introduced together with pRep (Qiagen) into SG22189 (Table II). The resulting strain was used to express and purify ClpP‐His6 as described previously for MecA purification (Turgay et al., 1997). To test the function of the ClpP‐His6 fusion in B.subtilis, the last 394 bp of the clpP gene, including the sequence encoding the His6‐tag fusion, were cloned in a pUC18 plasmid carrying a kanamycin‐resistance cassette. This construct, pK1, was used to transform B.subtilis BD2711 (Table II), to achieve a Campbell‐like integration at the clpP locus, inactivating the resident clpP gene and placing the clpP‐his6 fusion under the control of the clpP promoter. The resulting strain BD2725 was competent and expressed ComK‐green fluorescent protein (GFP) at the wild‐type level (not shown). ClpC, MecA, ComK and ComS were obtained as described previously (Turgay et al., 1997).
Calibrated Western blotting
All antibodies (Kong and Dubnau, 1994; Turgay et al., 1997) were immunoaffinity purified on protein‐coupled Affigel‐15 columns (Harlow and Lane, 1988). Bacillus subtilis IS75 cells were grown in competence medium (Albano et al., 1987) to t0, t1 and t2. To determine the number of cells in these samples, an aliquot was fixed with 0.37% formaldehyde and counted in a Petroff‐Hauser chamber. The cells were lysed in a French pressure cell and the extracts were boiled for 5 min in sample buffer (2% SDS, 125 mM Tris pH 6.8, 5% β‐mercaptoethanol, 15% glycerol, 0.005% bromophenol blue) and analyzed by Western blotting. On each gel, known amounts of purified ClpC, ComK or MecA were included to construct a standard curve. The concentrations of the protein stock solutions were determined by amino acid analysis (Protein Chemistry Facility, Columbia University).
Determination of in vivo ComK and MecA stabilities
The B.subtilis strains were grown in competence medium to t2 and diluted 1:20 into fresh competence medium with the addition of tetracycline (50 μg/ml) and rifampicin (5 μg/ml). The cultures were incubated at 37°C and 50 ml samples were taken. The cells were centrifuged, resuspended in 1 ml STM (50 mM NaCl, 25% sucrose, 50 mM Tris‐HCl pH 8, 5 mM MgCl2), washed and resuspended in STM + lysozyme (300 μg/ml). The cells were protoplasted by incubation at 37°C for 10 min. Sample buffer was added and the samples were boiled for 5 min. The remaining extract was used to determine protein concentration. The protein extracts (20 μg/lane) were analyzed by Western blotting. ComK was also investigated in the BD2524 background. In this case samples were harvested hourly during growth in competence medium containing xylose (2% w/v) and treated as described for the previous experiment. To test the stability of MecA in the wild‐type and mutant strains, cells were harvested at t2. Extracts were prepared and analyzed as described above.
In vitro degradation and ATPase assay
To determine the in vitro stability of ComK we used buffer A (100 mM KCl, 25 mM MOPS pH 7.0, 5 mM MgCl2, 0.5 mM DTT) containing 4 mM ATP and 2 mM PEP with 1.17 μM pyruvate kinase (Boehringer Mannheim). The assay was performed in final volumes of 50 μl and incubation was at 37°C. Seventeen microliters of 4× sample buffer was added to stop the reaction, the mixture was boiled for 5 min, and 3 μl was applied to a 12% SDS‐polyacrylamide gel and analyzed by Western blotting.
The in vitro stabilities of MecA and ComS were determined in buffer A containing 4 mM ATP, 2 mM PEP and 1.17 μM pyruvate kinase. The reaction volumes were 50 μl and samples were taken as described above for the ComK analysis. In the experiment shown in Figure 5, 10 μl aliquots were run on a Tricine (Figure 5A) or a 12% SDS‐polyacrylamide gel (Figure 5B) and stained with Coomassie Blue.
The ATPase assays were carried out in buffer A as described previously (Turgay et al., 1997).
ClpP (2 mg/ml) was dialyzed against 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4 and crosslinked to activated Affigel‐10 (Bio‐Rad) as recommended by the manufacturer. Two identical 200 microliter columns were poured with this material and washed extensively with buffer A with and without 2 mM ATP. ClpC (0.93 μM) in 200 μl of buffer A with and without ATP was loaded on each column and incubated for 80 min at room temperature. Two‐hundred microliters of buffer A was applied as the first wash, with or without ATP. The columns were then washed with a total of 5 ml of buffer A with or without ATP. A final additional wash of 200 μl was collected. Three 200 μl aliquots of the elution buffer (500 mM NaCl, 5 mM EDTA) were then applied and the eluates were collected. The columns were washed with an additional 1 ml of elution buffer, and then with a final aliquot of 200 μl which was collected. Ten microliters of each sample were analyzed by Western blotting.
We acknowledge valuable discussions with all the members of our laboratory and with L.Hamoen and S.Gottesman. We also thank L.Hamoen for providing ComK protein. We thank S.Gottesman, A.D.Grossman, U.Gerth, M.Hecker and L.Sonenshein for their generous gifts of strains. We thank U.Gerth and M.Hecker for providing the sequence of B.subtilis clpP, and T.Baker and Y.‐I.Kim for communicating information prior to publication. This work was supported by NIH grant AI10311.
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